Hernández G, Sierra J M
Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Biochim Biophys Acta. 1995 Apr 26;1261(3):427-31. doi: 10.1016/0167-4781(95)00039-j.
A Drosophila melanogaster cDNA clone encoding the translation initiation factor eIF-4E was isolated and sequenced. The deduced polypeptide consists of 259 amino acids with a predicted molecular weight of 29,223. It shares 48%, 37% and 35% identity to its mammalian, yeast and wheat counterparts, respectively. Several residues (including eight tryptophans), which were shown to be critical for the function of mammalian and yeast eIF-4Es, are conserved in the Drosophila protein. Three transcripts of the eIF-4E gene were detected throughout Drosophila development.
分离并测序了一个编码翻译起始因子eIF - 4E的黑腹果蝇cDNA克隆。推导的多肽由259个氨基酸组成，预测分子量为29223。它与哺乳动物、酵母和小麦的对应物分别有48%、37%和35%的同源性。几个对哺乳动物和酵母eIF - 4E功能至关重要的残基（包括八个色氨酸）在果蝇蛋白中是保守的。在果蝇的整个发育过程中检测到了eIF - 4E基因的三种转录本。
