Ye X, Cavener D R
Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235.
Gene. 1994 May 16;142(2):271-4. doi: 10.1016/0378-1119(94)90273-9.
Drosophila melanogaster cDNA clones encoding the beta subunit of translation initiation factor 2 (eIF-2) were isolated and sequenced. The longest cDNA predicts a protein of 312 amino acids (aa), which possesses a putative RNA-binding motif and a highly charged N-terminal region composed of three basic polylysine blocks. The aa sequence comparison of D. melanogaster eIF-2 beta with its human and yeast counterparts demonstrates a high degree of similarity, especially within the C-terminal region. Northern analysis indicates quasi-constitutive expression of eIF-2 beta throughout D. melanogaster development.
分离并测序了编码翻译起始因子2（eIF-2）β亚基的黑腹果蝇cDNA克隆。最长的cDNA预测编码一个312个氨基酸（aa）的蛋白质，该蛋白质具有一个推定的RNA结合基序和一个由三个碱性多聚赖氨酸块组成的高度带电的N端区域。黑腹果蝇eIF-2β与人类和酵母对应物的氨基酸序列比较显示出高度相似性，尤其是在C端区域。Northern分析表明eIF-2β在黑腹果蝇整个发育过程中呈准组成型表达。
