Ferreira A P, Faquim E S, Abrahamsohn I A, Macedo M S
Department of Immunology, University of São Paulo, Brazil.
Cell Immunol. 1995 May;162(2):202-10. doi: 10.1006/cimm.1995.1070.
The effect of an Ascaris suum extract (Asc) on several T cell functions was studied in mice immunized with Asc and ovalbumin (OA) in complete Freund's adjuvant. Delayed-type hypersensitivity reactions following challenge with aggregated OA were markedly diminished in mice injected with OA plus a high dose of Asc compared to OA-immunized animals. Proliferation and IL-2, IFN-gamma, IL-4, and IL-10 production in OA-stimulated lymph node cells from the OA + Asc-immunized group were also inhibited. Titration of anti-OA antibodies also showed suppression of IgG1, IgG2a, and IgE isotypes in the animals injected simultaneously with the extract. The degree of suppression induced by Asc on OA-specific cell-mediated responses was dose dependent. The profile of cytokines synthesized in response to Asc also changed depending on the injected dose. IL-4 and IL-10 were mainly produced in response to high doses, whereas IL-2 and IFN-gamma were greatly enhanced at a low dose of Asc. These findings indicate that the A. suum extract may impair crucial T cell functions for cell-mediated as well as humoral immune responses to other antigens through the induction of a predominantly Th2-like response.
在以完全弗氏佐剂中的猪蛔虫提取物（Asc）和卵清蛋白（OA）免疫的小鼠中，研究了猪蛔虫提取物对几种T细胞功能的影响。与仅用OA免疫的动物相比，用OA加高剂量Asc注射的小鼠在受到聚集的OA攻击后，迟发型超敏反应明显减弱。来自OA + Asc免疫组的OA刺激的淋巴结细胞中的增殖以及IL-2、IFN-γ、IL-4和IL-10的产生也受到抑制。抗OA抗体的滴定还显示，同时注射提取物的动物中IgG1、IgG2a和IgE同种型受到抑制。Asc对OA特异性细胞介导反应的抑制程度呈剂量依赖性。响应Asc合成的细胞因子谱也根据注射剂量而变化。高剂量主要产生IL-4和IL-10，而低剂量的Asc则大大增强IL-2和IFN-γ的产生。这些发现表明，猪蛔虫提取物可能通过诱导主要为Th2样反应，损害对其他抗原的细胞介导以及体液免疫反应的关键T细胞功能。
