Bryant G
Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen, Federal Republic of Germany.
Cryobiology. 1995 Apr;32(2):114-28. doi: 10.1006/cryo.1995.1011.
The formation of intracellular ice in biological cells during freezing is considered almost universally lethal and is the major contributor to cell damage at high cooling rates. Despite its importance, our understanding of the mechanisms of intracellular ice formation (IIF) is still incomplete. In this paper differential scanning calorimetry is used to study IIF in human lymphocytes in the presence of dimethyl sulfoxide (Me2SO). Under conditions where damage due to IIF on the initial cooling run is 40-60%, the samples are studied as a function of multiple successive cooling runs. This enables the study not only of the cell fraction which undergoes IIF, but also of the fraction which survives. The temperature at which IIF occurs and the fraction of cell volume which undergoes IIF are analyzed as functions of successive cooling runs. Taking advantage of the large number of cells present in the samples (ca. 10(6)), the effect of successive cooling runs on susceptibility to IIF is examined.
在冷冻过程中，生物细胞内形成的细胞内冰几乎被普遍认为是致命的，并且是高冷却速率下细胞损伤的主要原因。尽管其重要性，但我们对细胞内冰形成（IIF）机制的理解仍然不完整。本文利用差示扫描量热法研究了在二甲基亚砜（Me2SO）存在下人类淋巴细胞中的IIF。在初始冷却过程中由于IIF造成40%-60%损伤的条件下，对样品进行多次连续冷却过程的研究。这不仅能够研究经历IIF的细胞部分，还能研究存活的部分。分析IIF发生的温度以及经历IIF的细胞体积分数与连续冷却过程的关系。利用样品中存在的大量细胞（约10^6个），研究连续冷却过程对IIF易感性的影响。
