Nir-Paz R, Israel S, Honigman A, Kahane I
Department of Membrane, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
FEMS Microbiol Lett. 1995 Apr 15;128(1):63-8. doi: 10.1111/j.1574-6968.1995.tb07501.x.
Mycoplasmas have been incriminated in setting the stage for HIV infection and full-blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T-cells, transfected with plasmids harboring a transcription fusion construct between HIV-long terminal repeat (HIV-LTR) and either luc or cat genes, were infected with several mycoplasmas (M. fermentans; M. penetrans, M. pirum and Ureaplasma urealyticum) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV-LTR mediated transcription.
支原体被认为在为HIV感染和全面发展的艾滋病创造条件方面起了作用。我们测试了支原体在HIV激活过程中可能的参与情况。用携带HIV长末端重复序列（HIV-LTR）与荧光素酶（luc）或氯霉素乙酰转移酶（cat）基因之间转录融合构建体的质粒转染的两种细胞系，即293成纤维细胞和Jurkat CD4 + T细胞，被几种支原体（发酵支原体；穿透支原体、梨支原体和解脲脲原体）感染，并监测报告基因的表达。此处呈现的数据表明，支原体，尤其是它们的膜，在HIV-LTR介导的转录激活中发挥作用。
