Metzger K, Angres G, Maier H, Lehmann W D
Central Spectroscopy Department, German Cancer Research Center, Heidelberg.
Free Radic Biol Med. 1995 Feb;18(2):185-94. doi: 10.1016/0891-5849(94)00108-v.
Lipoxygenase products were quantified in human mixed saliva and in saliva fractions obtained from a parotid or submandibular gland using gas chromatography-mass spectrometry and stable isotope dilution. In glandular saliva, only linoleic acid was detected at levels of 20-30 ng/ml. In contrast, mixed saliva showed a linoleic acid concentration of around 300 ng/ml, arachidonic acid levels of around 30 ng/ml, hydroxyoctadecadienoic acid (HODE) levels between 5 and 10 ng/ml, and hydroxyeicosatetraenoic acid (HETE) levels up to 25 ng/ml. By far the most abundant HETE was 12-HETE, and incubation experiments with arachidonic acid showed the presence of a substantial 12-lipoxygenase activity in human mixed saliva, but not in saliva fractions. This activity was identified as 12(S)-lipoxygenase activity by chiral analysis of the reaction product. Investigating mixed saliva and glandular saliva of patients with squamous cell carcinoma in the upper aerodigestive tract and of controls, most patients showed elevated levels of free arachidonic acid and elevated HETE levels. Besides a moderate increase in 12-HETE levels, markedly elevated concentrations of 5-HETE and 15-HETE were observed for the carcinoma patients. The level of free arachidonic acid and the quantitative HETE profile appear to be good markers for the inflammatory processes occurring in the oral mucosa and in saliva in response to the development of squamous cell carcinoma.
使用气相色谱 - 质谱联用和稳定同位素稀释法对人混合唾液以及从腮腺或颌下腺获得的唾液组分中的脂氧合酶产物进行定量。在腺性唾液中，仅检测到亚油酸，水平为20 - 30 ng/ml。相比之下，混合唾液中亚油酸浓度约为300 ng/ml，花生四烯酸水平约为30 ng/ml，羟基十八碳二烯酸（HODE）水平在5至10 ng/ml之间，羟基二十碳四烯酸（HETE）水平高达25 ng/ml。到目前为止，最丰富的HETE是12 - HETE，花生四烯酸的孵育实验表明人混合唾液中存在大量的12 - 脂氧合酶活性，但在唾液组分中不存在。通过对反应产物的手性分析，该活性被鉴定为12(S) - 脂氧合酶活性。对患有上呼吸道消化道鳞状细胞癌的患者和对照组的混合唾液及腺性唾液进行研究，大多数患者显示游离花生四烯酸水平升高和HETE水平升高。除了12 - HETE水平适度增加外，癌患者还观察到5 - HETE和15 - HETE浓度明显升高。游离花生四烯酸水平和定量的HETE谱似乎是口腔黏膜和唾液中因鳞状细胞癌发展而发生的炎症过程的良好标志物。
