He J, Li Y, Gao X
Hua Xi Yi Ke Da Xue Xue Bao. 1994 Dec;25(4):452-5.
An enzyme-linked immunoassay (EIA) for testosterone was developed based on penicillinase as the marker enzyme. The assay proposed requires a conjugate containing penicillinase as the enzymatic component, penicillin Vas substrate and an iodine-starch reagent as the chromogenic component. The binding of conjugate molecules results in the transition of the iodic complex from dark blue to colourless. delta A (the difference between the blank and the testosterone) vs log C was linear over the range 10(-7) - 10(-2)mg/ml (r = 0.9969), with a limit of detection 25 pg per disc. The CVs of day to day and within day for plasma testosterone were < 3.3% and 5.9%, respectively. There was a good correlation between the values obtained by EIA and radioimmunoassay (r = 0.937).
基于青霉素酶作为标记酶开发了一种睾酮酶联免疫分析（EIA）方法。所提出的分析方法需要一种含有青霉素酶作为酶成分、青霉素V作为底物以及碘 - 淀粉试剂作为显色成分的结合物。结合物分子的结合导致碘络合物从深蓝色转变为无色。在10（-7） - 10（-2）mg/ml范围内，ΔA（空白与睾酮之间的差值）与log C呈线性关系（r = 0.9969），检测限为每片25 pg。血浆睾酮的日间和日内变异系数分别<3.3%和5.9%。EIA法与放射免疫分析法所得值之间具有良好的相关性（r = 0.937）。
