Joshi U M, Shah H P, Sudhama S P
Steroids. 1979 Jul;34(1):35-46. doi: 10.1016/0039-128x(79)90124-7.
A very sensitive enzymeimmunoassay for testosterone was developed using testosterone-penicillinase conjugate and an antibody to testosterone-3-(O-carboxymethyl)oxime-bovine serum albumin. The specificity of the assay was demonstrated by the fact that estradiol-17 beta, estrone, estriol, progesterone, 17 alpha-hydroxy-progesterone, dehydroepiandrosterone, androstenedione, cortisol and cortisone were ineffective in crossreacting with testosterone while dihydrotestosterone was 8 times less crossreactive as compared to testosterone. The minimum detectable amount of testosterone was 10-15 pg per assay tube. Intra-assay and inter-assay coefficients of variation for samples containing 0.3-6ng/ml of testosterone were 6-8% and 8-10%, respectively. A high degree of correlation (r = 0.97) was observed between serum testosterone values obtained by enzymeimmunoassay and radioimmunoassay. The levels of testosterone in the sera of normal men and women and those in hypogonadal males following stimulation with human chorionic gonadotropin determined by this enzymeimmunoassay appear similar to those reported by other investigators.
利用睾酮 - 青霉素酶偶联物和抗睾酮 - 3 -（O - 羧甲基）肟 - 牛血清白蛋白抗体，开发了一种用于检测睾酮的高灵敏度酶免疫测定法。该测定法的特异性通过以下事实得以证明：雌二醇 - 17β、雌酮、雌三醇、孕酮、17α - 羟基孕酮、脱氢表雄酮、雄烯二酮、皮质醇和可的松与睾酮无交叉反应，而双氢睾酮与睾酮相比交叉反应性低8倍。每个测定管中睾酮的最低可检测量为10 - 15皮克。含0.3 - 6纳克/毫升睾酮的样本的批内和批间变异系数分别为6 - 8%和8 - 10%。酶免疫测定法和放射免疫测定法测得的血清睾酮值之间观察到高度相关性（r = 0.97）。通过这种酶免疫测定法测定的正常男性和女性血清以及性腺功能减退男性经人绒毛膜促性腺激素刺激后的睾酮水平，与其他研究者报告的水平相似。
