Yip I, Pang X P, Berg L, Hershman J M
Endocrine Research Laboratory, West Los Angeles Veterans Affairs Medical Center, California 90073, USA.
J Clin Endocrinol Metab. 1995 May;80(5):1664-9. doi: 10.1210/jcem.80.5.7745015.
To test the hypothesis that interferon-gamma (IFN gamma) and interleukin-1 beta (IL-1 beta) possess antitumor activity on human papillary thyroid carcinoma cells, we studied the in vitro effects of IFN gamma and IL-1 beta on the proliferation and invasiveness of two human PTC cell lines, TPC-1 (TP) and NPA (NP) cells. TP and NP cells were treated with various concentrations of IFN gamma and IL-1 beta alone and in combination. Cell proliferation was assessed by [3H]thymidine incorporation and cell number measurement. Tumor cell invasion was assessed by the ability of cells to penetrate through a Matrigel membrane. Both IFN gamma and IL-1 beta inhibited [3H]thymidine incorporation into TP cells in a dose-dependent manner and decreased TP cell number. In NP cells, treatment with IFN gamma and IL-1 beta also decreased [3H]thymidine incorporation and cell number. The inhibitory effects of IFN gamma and IL-1 beta on tumor cell proliferation were additive in both cell lines. In the invasion experiments, IFN gamma and IL-1 beta reduced the invasiveness of TP and NP cells. Again, the inhibitory effects of IFN gamma and IL-1 beta on tumor cell invasion were additive in both cell lines. In summary, the results showed that both IFN gamma and IL-1 beta are potent inhibitors of the proliferation and invasiveness of TP and NP cells. The additive effects of IFN gamma and IL-1 beta are evidence that they act through different pathways. Our findings suggest that IFN gamma and IL-1 beta are two of the anticancer factors that act to suppress the proliferation and reduce the invasive potential of human papillary thyroid carcinoma cells.
为了验证γ干扰素（IFNγ）和白细胞介素-1β（IL-1β）对人甲状腺乳头状癌细胞具有抗肿瘤活性这一假说，我们研究了IFNγ和IL-1β对两种人甲状腺乳头状癌（PTC）细胞系TPC-1（TP）和NPA（NP）细胞增殖和侵袭能力的体外影响。单独及联合使用不同浓度的IFNγ和IL-1β处理TP和NP细胞。通过[3H]胸腺嘧啶核苷掺入法和细胞计数评估细胞增殖。通过细胞穿透基质胶膜的能力评估肿瘤细胞侵袭。IFNγ和IL-1β均以剂量依赖方式抑制[3H]胸腺嘧啶核苷掺入TP细胞，并减少TP细胞数量。在NP细胞中，用IFNγ和IL-1β处理也降低了[3H]胸腺嘧啶核苷掺入和细胞数量。IFNγ和IL-1β对肿瘤细胞增殖的抑制作用在两种细胞系中均具有相加性。在侵袭实验中，IFNγ和IL-1β降低了TP和NP细胞的侵袭能力。同样，IFNγ和IL-1β对肿瘤细胞侵袭的抑制作用在两种细胞系中也具有相加性。总之，结果表明IFNγ和IL-1β均是TP和NP细胞增殖和侵袭的有效抑制剂。IFNγ和IL-1β的相加作用证明它们通过不同途径发挥作用。我们的研究结果表明，IFNγ和IL-1β是两种抗癌因子，可抑制人甲状腺乳头状癌细胞的增殖并降低其侵袭潜能。
