Tigano-Milani M S, Honeycutt R J, Lacey L A, Assis R, McClelland M, Sobral B W
Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (CENARGEN), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA).
J Invertebr Pathol. 1995 May;65(3):274-82. doi: 10.1006/jipa.1995.1042.
Paecilomyces fumosoroseus (Deuteromycotina:Hyphomycetes) is a fungus that is potentially useful for the bio-control of economically important agricultural pests, such as whitefly (Bemisia tabaci). Arbitrarily primed PCR and PCR with tRNA consensus primers were used to analyze genetic variability among 27 P. fumosoroseus isolates, 15 of which came from the same host, B. tabaci, one P. lilacinus isolate, used as an outgroup, 9 previously unidentified Paecilomyces isolates. Fifteen 10-mer oligonucleotide primers of arbitrary sequence revealed 322 scorable binary characters. Principal coordinates and cluster analysis of characters showed that most of the P. fumosoroseus and Paecilomyces sp. isolates were in three phenetic groups with > 65% internal similarity. Two of the three arbitrary phenetic groups were closely related (76% similarity) with the third group quite different (only 14% similarity) from the first two. The phenetic groups did not correlate with geographical origin or host species. Genetic variability of isolates infecting whitefly in Florida was detected. Isolates from B. tabaci were represented in two of the three groups, and different genotypes were identified even when they were isolated from an epizootic population in India and Pakistan. There was no evidence of host-specific selection of genotypes, as has been shown in other entomopathogenic fungi. Three isolates morphologically classified as P. fumosoroseus were clustered in a phenetic group which displayed only 14% similarity to the other isolates of this species. Seven isolated that presented problems for morphological classification were found to be similar or, in three cases, identical to other P. fumosoroseus isolates that dit not present problems for morphological classification.(ABSTRACT TRUNCATED AT 250 WORDS)
玫烟色拟青霉（半知菌亚门：丝孢纲）是一种真菌，对经济上重要的农业害虫如粉虱（烟粉虱）的生物防治具有潜在用途。采用随机引物PCR和tRNA共有引物PCR分析了27株玫烟色拟青霉分离株的遗传变异性，其中15株来自同一宿主烟粉虱，1株淡紫拟青霉分离株用作外群，9株为先前未鉴定的拟青霉分离株。15个任意序列的10聚体寡核苷酸引物揭示了322个可计分的二元性状。性状的主坐标和聚类分析表明，大多数玫烟色拟青霉和拟青霉属分离株分在三个表型组中，组内相似性>65%。三个任意表型组中的两个密切相关（相似性76%），而第三个组与前两个组差异很大（仅14%相似性）。这些表型组与地理起源或宿主物种无关。检测到佛罗里达州感染粉虱的分离株的遗传变异性。来自烟粉虱的分离株分布在三个组中的两个组中，即使从印度和巴基斯坦的一个 epizootic 种群中分离出来，也鉴定出了不同的基因型。没有证据表明存在如其他昆虫病原真菌中所显示的宿主特异性基因型选择。三个形态学上归类为玫烟色拟青霉的分离株聚在一个表型组中，该组与该物种的其他分离株仅显示14%的相似性。发现7个在形态学分类上存在问题的分离株与其他在形态学分类上没有问题的玫烟色拟青霉分离株相似，在3个案例中相同。（摘要截短于250字）
