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来自墨西哥的新物种(真菌: Orbili 目):其液体培养滤液对捻转血矛线虫(线虫纲:毛圆科)的捕食活性和杀线虫活性

sp. nov. (Fungi: Orbiliales) from Mexico: Predatory Activity and Nematocidal Activity of Its Liquid Culture Filtrates Against (Nematoda: Trichostrongylidae).

作者信息

Gutiérrez-Medina Enrique, Mendoza-de Gives Pedro, Pérez-Anzúrez Gustavo, Colinas-Picazo Antonio, Bautista-García Génesis Andrea, Alonso-Díaz Miguel Ángel, von Son-de Fernex Elke, López-Arellano María Eugenia

机构信息

Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock (INIFAP-AGRICULTURA), Jiutepec 62550, Mexico.

Production Sciences and Animal Health, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico (UNAM), Coyoacán, Mexico City 04510, Mexico.

出版信息

J Fungi (Basel). 2024 Dec 22;10(12):888. doi: 10.3390/jof10120888.

Abstract

During the isolation, identification, and assessment of nematode-trapping fungi (NTF) against nematodes, we discovered an unusual fungus in decaying wood from Morelos State, Mexico. This isolate exhibited some characteristics similar to those of the genus; however, we found that it did not match any previously reported species within this genus after conducting morphological and phylogenetic analyses using the ITS, TEF, and RPB2 regions. This new species displays conidiophores with two or three stems emerging from the same initial site and conidiophores with only a single stem and aerial thickened hyphae from which single conidiophores emerge, forming 3D adhesive nets. The conidia, which have one or two septa, range from obovoid to ellipsoidal, crowned by four to six conidia. This report provides evidence that this species has not been described before, and we hereby introduce it as a new species, naming it . This species displayed a predatory activity of 76.92%, and its liquid culture filtrates in Sweet Potato Dextrose Broth and Czapek-Dox Broth were effective in killing 40.90% and 34.91% of larvae, respectively. This study provides information about a previously unreported species of nematophagous fungus, which is important for systematics and has potential biotechnological applications against nematodes that affect the livestock industry.

摘要

在对线虫捕食真菌(NTF)进行分离、鉴定和评估以对抗线虫的过程中,我们在墨西哥莫雷洛斯州腐烂木材中发现了一种不寻常的真菌。该分离株表现出一些与该属相似的特征;然而,在使用ITS、TEF和RPB2区域进行形态学和系统发育分析后,我们发现它与该属内任何先前报道的物种都不匹配。这个新物种展示出分生孢子梗,有两个或三个茎从同一初始位点长出,还有只有单个茎的分生孢子梗以及气生加厚菌丝,单个分生孢子梗从气生加厚菌丝上长出,形成三维粘性网。分生孢子有一个或两个隔膜,形状从倒卵形到椭圆形不等,顶部有四到六个分生孢子。本报告提供了该物种此前未被描述的证据,我们在此将其作为一个新物种引入,并命名为 。该物种的捕食活性为76.92%,其在甘薯葡萄糖肉汤和察氏肉汤中的液体培养滤液分别能有效杀死40.90%和34.91%的 幼虫。本研究提供了一种此前未报道的食线虫真菌物种的信息,这对于分类学很重要,并且在对抗影响畜牧业的线虫方面具有潜在的生物技术应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f30/11678410/51e434bfeca0/jof-10-00888-g001.jpg

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