Assessing the In Vitro Individual and Combined Effect of and (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode (Trichostrongylidae).

BACKGROUND

species are nematophagous fungi that secrete extracellular nematocidal products (ECP). The individual and combined effects of ECP from () and () growth in liquid media against L3 (HcL3) were assessed.

METHODS

The isolation, morphological (MI) and molecular identification (Mol-I), assessment of nematocidal activity (NA) of fungal liquid culture filtrates (LCF) in two liquid media alone and in combination and the myco-compound profile identification (MCP) were performed.

RESULTS

The MI suggested that the fungi corresponded to the species and . This result was confirmed by PCR analysis followed by sequencing, alignment and a phylogenetic analysis. Likewise, the highest mortalities were 91.4% with individual LCF of and 86.2% with those of at the highest concentration (100 mg/mL) in Czapek-Dox Broth. The combination of both LCF resulted in a similarly high larval mortality with no statistical differences in relation to individual activity ( > 0.05). The MCP showed the presence of alkaloids in both fungi. Coumarins, sterols and saponins were found only in .

MAIN CONCLUSIONS

Both fungi produced ECP with a high NA that could be identified and assessed in future studies as potential natural anthelmintic compounds.