Suzuki J, Yoshihara E, Kobayashi S, Fukuyama M, Kishikawa S, Hara M, Ikeda T, Tabuchi K
Department of Bioorganic Chemistry, Azabu University.
Kansenshogaku Zasshi. 1995 Mar;69(3):327-32. doi: 10.11150/kansenshogakuzasshi1970.69.327.
The hemolysin produced by group C streptococci (GCH) has an isoelectric point (pI) of 5.6 and a molecular weight of 32,000 and shows hemolytic activity in the absence of 2-mercaptoethanol (2-ME). The hemolytic activity of GCH was compared to that of streptolysin S (SLS). These hemolysins did not differ with respect to the binding and release of hemoglobin (Hb). GCH was bound to phospholipids on the membranes of target erythrocytes, followed by the rapid released of K+ and slow Hb release after a relatively long lag time. GCH showed a lower hemolytic efficiency than SLS, reflecting the fact that these hemolysins destroy erythrocytes by slightly different mechanisms.
C 组链球菌（GCH）产生的溶血素的等电点（pI）为5.6，分子量为32,000，且在没有2-巯基乙醇（2-ME）的情况下显示溶血活性。将GCH的溶血活性与链球菌溶血素S（SLS）的溶血活性进行了比较。这些溶血素在血红蛋白（Hb）的结合和释放方面没有差异。GCH与靶红细胞膜上的磷脂结合，随后K+迅速释放，经过相对较长的延迟时间后Hb缓慢释放。GCH的溶血效率低于SLS，这反映了这些溶血素通过略有不同的机制破坏红细胞这一事实。
