Fukuyama M, Suda O, Hara M, Kishikawa S, Suzuki J, Ikeda T, Tabuchi K, Imagawa Y
Department of Environmental Microbiology, Azabu University.
Kansenshogaku Zasshi. 1993 Dec;67(12):1155-9. doi: 10.11150/kansenshogakuzasshi1970.67.1155.
Between mid-October to mid-November 1992, of 500 freely-ranging Formosan and striped squirrels kept at Garden Y in the suburbs of Kanagawa Prefecture, 414 (82.8%) suddenly died one after another by bleeding from the nasal and oral cavities after developing a mild facial swelling. Isolation of microbes including viruses were carried out from the Formosan squirrels that had suddenly died. Various organs from these animals were histologically examined. 1. In bacteriological tests, beta-hemolytic streptococcal strains were isolated in a pure culture from 5 (83.3%) of 6 Formosan squirrels that had died suddenly. By serological analysis, 14 isolated strains were serotyped as group C according to the classification of Lancefield. From their biochemical characteristics, these were identified as Streptococcus equi subsp. zooepidemicus. A drug sensitivity test revealed that ABPC, PCG, SBPC, CMX and CPZ are highly sensitive against the isolates. 2. In the virological test, the viral isolation was applied for three blind passages by primary cultured kidney cells of Formosan squirrels, but no evidence of CPE was obtained. 3. At autopsy, a pathological change was detected mainly in the lungs. Histopathological examinations revealed severe hypertrophic changes of the alveolar wall in the entire pulmonary lobe. Severe congestion, hemorrhagic pneumonia, neutrophils and macrophages infiltration were observed in the hypertrophic alveolar wall. In the other cases, thrombi were observed in the branches of the pulmonary artery. Other organs demonstrated no remarkable histopathological changes. 4. Streptococcal strains were not isolated from the pharynx in all of the employees working at this garden.
1992年10月中旬至11月中旬，在神奈川县郊区的Y花园中饲养的500只自由放养的台湾松鼠和条纹松鼠中，有414只（82.8%）在出现轻微面部肿胀后，相继突然死于鼻腔和口腔出血。对突然死亡的台湾松鼠进行了包括病毒在内的微生物分离。对这些动物的各种器官进行了组织学检查。1. 在细菌学检测中，从6只突然死亡的台湾松鼠中的5只（83.3%）的纯培养物中分离出β-溶血性链球菌菌株。通过血清学分析，根据兰斯菲尔德分类法，14株分离菌株被鉴定为C组。根据其生化特性，这些菌株被鉴定为马链球菌兽疫亚种。药敏试验表明，ABPC、PCG、SBPC、CMX和CPZ对分离株高度敏感。2. 在病毒学检测中，用台湾松鼠的原代培养肾细胞进行了三次盲传病毒分离，但未获得细胞病变效应的证据。3. 尸检时，主要在肺部检测到病理变化。组织病理学检查显示整个肺叶的肺泡壁严重肥大。在肥大的肺泡壁中观察到严重充血、出血性肺炎、中性粒细胞和巨噬细胞浸润。在其他病例中，在肺动脉分支中观察到血栓。其他器官未显示明显的组织病理学变化。4. 在该花园工作的所有员工的咽部均未分离出链球菌菌株。
