Jacoby R O, Johnson E A, Ball-Goodrich L, Smith A L, McKisic M D
Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8016, USA.
J Virol. 1995 Jun;69(6):3915-9. doi: 10.1128/JVI.69.6.3915-3919.1995.
Infection of young adult BALB/cByJ mice with mouse parvovirus-1, a newly recognized, lymphocytotropic, nonpathogenic parvovirus, was examined by in situ hybridization. Virus appeared to enter through the small intestine and was disseminated to the liver and lymphoid tissues. Strand-specific probes detected virion DNA in a consistently larger number of cells than replicative forms of viral DNA and/or viral mRNA. The number of signal-positive cells in the intestinal mucosa, lymph nodes, spleen, and thymus increased through day 10 after oral inoculation but decreased after seroconversion. Positive cells were still detected, however, in peripheral lymphoid tissues of mice examined at 9 weeks postinoculation. The results underscore the need to assess potential effects of persistent mouse parvovirus-1 infection on immune function in mice.
通过原位杂交技术研究了新型嗜淋巴细胞性非致病性小鼠细小病毒1对年轻成年BALB/cByJ小鼠的感染情况。病毒似乎通过小肠进入，并扩散到肝脏和淋巴组织。链特异性探针检测到的病毒粒子DNA数量始终比病毒DNA复制形式和/或病毒mRNA的数量更多。口服接种后第10天，肠道黏膜、淋巴结、脾脏和胸腺中信号阳性细胞的数量增加，但血清转化后减少。然而，在接种后9周检查的小鼠外周淋巴组织中仍可检测到阳性细胞。这些结果强调了评估持续性小鼠细小病毒1感染对小鼠免疫功能潜在影响的必要性。
