唾液肽P-C中的必需氨基酸结构域可增强葡萄糖诱导的胰岛素释放，并抑制精氨酸诱导的灌注大鼠胰腺中胰高血糖素的释放。

The essential amino acid domains in salivary peptide P-C that potentiate glucose-induced insulin release and inhibit arginine-induced glucagon release from perfused rat pancreas.

作者信息

Kimura M, Nakashima N, Kimura I

机构信息

Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.

出版信息

Jpn J Pharmacol. 1995 Jan;67(1):79-82. doi: 10.1254/jjp.67.79.

DOI:10.1254/jjp.67.79

PMID:7745848

Abstract

The amino acid domains in salivary peptide P-C (1-44 peptide fragments, P-C) that are essential to potentiate insulin release and inhibit glucagon release were investigated using isolated perfused rat pancreas. P-C significantly potentiated not only glucose (8.3 mM)-induced insulin release, but also arginine (10 mM)-induced insulin release. The essential domain responsible for potentiation of insulin release was the P-C-(23-29) fragment, 23KPQGPPP29, while that inhibiting glucagon release was the P-C-(12-18) fragment, 12HQQGPPP18. Since both domains share a common fragment, QGPP, these findings indicate that the functional amino acid sequences KP and HQ may potentiate insulin release and inhibit glucagon release, respectively.

摘要

利用离体灌注大鼠胰腺，研究了唾液肽P-C（1-44肽片段，P-C）中对增强胰岛素释放和抑制胰高血糖素释放至关重要的氨基酸结构域。P-C不仅显著增强了葡萄糖（8.3 mM）诱导的胰岛素释放，还增强了精氨酸（10 mM）诱导的胰岛素释放。负责增强胰岛素释放的关键结构域是P-C-(23-29)片段，即23KPQGPPP29，而抑制胰高血糖素释放的结构域是P-C-(12-18)片段，即12HQQGPPP18。由于这两个结构域共享一个共同片段QGPP，这些发现表明功能性氨基酸序列KP和HQ可能分别增强胰岛素释放和抑制胰高血糖素释放。