Perez M T, Caminos E
Department of Ophthalmology, University Hospital of Lund, Sweden.
Neurosci Lett. 1995 Jan 2;183(1-2):96-9. doi: 10.1016/0304-3940(94)11123-z.
The expression of mRNA coding for brain-derived neurotrophic factor (BDNF) and for its functional receptor, the full-length tyrosine kinase receptor trkB (trkB mRNA), was examined in early postnatal and adult rat retina by in situ hybridization using digoxygenin and radioactively-labeled oligonucleotide probes. BDNF and trkB mRNAs are expressed in the ganglion cell layer at postnatal-days (PN) 1, 4, 7, 14, 60, in proximal neuroblastic layer (PN 1, 4, 7), and proximal inner nuclear layer (PN 14, 60). Subpopulations of developing and mature retinal cells are thus capable of synthesizing BDNF.
利用地高辛和放射性标记的寡核苷酸探针，通过原位杂交技术检测了出生后早期和成年大鼠视网膜中编码脑源性神经营养因子（BDNF）及其功能性受体全长酪氨酸激酶受体trkB（trkB mRNA）的mRNA表达。BDNF和trkB mRNA在出生后第1、4、7、14、60天的神经节细胞层、出生后第1、4、7天的近端成神经细胞层以及出生后第14、60天的近端内核层均有表达。因此，发育中和成熟的视网膜细胞亚群能够合成BDNF。
