Kanavaros P, Sakalidou A, Tzardi M, Darivianaki K, Delides G, Kazlaris E, Kalmanti M
Department of Pathology, University Hospital of Heraklion Crete, Greece.
Pathol Res Pract. 1994 Nov;190(11):1026-30. doi: 10.1016/s0344-0338(11)80897-6.
Paraffin sections from 22 cases of Hodgkin's disease (HD) and 30 cases of non-Hodgkin's lymphomas (NHL) occurring in childhood (3-15 years old) were examined for the presence of Epstein-Barr Virus (EBV) encoded EBER mRNAS and Latent Membrane Protein-1 (LMP-1) using RNA in situ hybridization (RISH) and immunohistochemistry, respectively. In 12/22 (54%) cases of HD the EBER transcripts were detected in most Reed-Sternberg and Hodgkin (HRS) cells as well as in some scattered smaller lymphoid cells. In all these cases the LMP-1 protein was detected exclusively in HRS cells. Three additional cases of HD were found to be EBER RISH positive only in a few scattered small lymphoid cells, the LMP-1 staining being negative in these cases. The EBER and LMP-1 positivity in HRS cells were present in 0/1 of lymphocyte predominant, 4/10 (40%) of nodular sclerosis and 8/11 (72%) of mixed cellularity of HD. No EBER RISH signal was found in tumor cells of the 30 cases of NHL. In four of them only a few scattered small lymphoid cells were EBER RISH positive. LMP-1 reactivity was not detected in any NHL. These results provide evidence for an association between EBV and a sizeable proportion of childhood Hodgkin's disease and show that this association is more frequent in mixed cellularity subtype. Furthermore, the detection of the LMP-1 protein in HRS cells in view of the LMP-1 transforming potential, suggests that EBV may be involved in the pathogenesis of a substantial proportion of cases of HD occurring in childhood.
对22例霍奇金淋巴瘤（HD）和30例儿童期（3至15岁）非霍奇金淋巴瘤（NHL）的石蜡切片，分别采用RNA原位杂交（RISH）和免疫组化检测爱泼斯坦-巴尔病毒（EBV）编码的EBER mRNA和潜伏膜蛋白-1（LMP-1）的表达情况。在22例HD中，12例（54%）的大多数里德-斯腾伯格细胞和霍奇金（HRS）细胞以及一些散在的较小淋巴细胞中检测到EBER转录本。在所有这些病例中，LMP-1蛋白仅在HRS细胞中检测到。另外3例HD仅在少数散在的小淋巴细胞中EBER RISH呈阳性，这些病例中LMP-1染色为阴性。HD的淋巴细胞为主型中0/1例、结节硬化型中4/10例（40%）、混合细胞型中8/11例（72%）的HRS细胞中存在EBER和LMP-1阳性。30例NHL的肿瘤细胞中未发现EBER RISH信号。其中4例仅少数散在的小淋巴细胞EBER RISH呈阳性。在任何NHL中均未检测到LMP-1反应性。这些结果为EBV与相当比例的儿童霍奇金淋巴瘤之间的关联提供了证据，并表明这种关联在混合细胞型亚型中更为常见。此外，鉴于LMP-1的转化潜能，在HRS细胞中检测到LMP-1蛋白表明EBV可能参与了相当比例儿童期HD病例的发病机制。
