Kontiainen S, Sivonen A, Renkonen O V
Department of Medical Microbiology, Aurora Hospital, Helsinki, Finland.
Scand J Infect Dis. 1994;26(6):685-91. doi: 10.3109/00365549409008636.
Yersinia strains recovered from routine stool cultures (n = 13,534) as well as clinical symptoms in the patients were analysed in order to establish whether enteric Yersinia enterocolitica strains recovered only after cold enrichment cultures shared the same pathogenicity markers and caused the same symptoms as strains from primary cultures. 93% of the 201 Yersinia isolates were Y. enterocolitica strains and 71% of these were of serotype O3. Nearly 40% of all Y. enterocolitica strains and 21% of serotype O3 strains were isolated only after 1 week's cold enrichment of stool specimens. Practically all Y. enterocolitica O3 strains, whether from primary or cold enrichment cultures, were pathogenic not only on the basis of the serotype but also on the basis of Congo-red uptake and calcium-dependent growth at 35 degrees C (CR-MOX test). The symptoms in patients with Y. enterocolitica O3 from primary and cold enrichment cultures were similar except that abdominal pains were more frequent (p < 0.05) in the former. Arthropathia, mesenteric lymphadenitis and erythema nodosum were detected in 15% of the patients with Yersinia isolates and were more frequent in patients with isolates from cold enrichment or without diarrhea.
对从常规粪便培养物中分离出的耶尔森氏菌菌株（n = 13,534）以及患者的临床症状进行了分析，以确定仅在冷增菌培养后分离出的肠道小肠结肠炎耶尔森氏菌菌株是否与初代培养物中的菌株具有相同的致病标记并引起相同的症状。在201株耶尔森氏菌分离株中，93%为小肠结肠炎耶尔森氏菌菌株，其中71%为O3血清型。几乎40%的小肠结肠炎耶尔森氏菌菌株和21%的O3血清型菌株仅在粪便标本冷增菌1周后才分离出来。实际上，所有小肠结肠炎耶尔森氏菌O3菌株，无论来自初代培养还是冷增菌培养，不仅基于血清型，而且基于刚果红摄取和35℃下的钙依赖性生长（CR-MOX试验）均具有致病性。来自初代培养和冷增菌培养的小肠结肠炎耶尔森氏菌O3患者的症状相似，只是前者腹痛更为常见（p < 0.05）。在15%的耶尔森氏菌分离株患者中检测到关节病、肠系膜淋巴结炎和结节性红斑，在冷增菌分离株患者或无腹泻患者中更为常见。
