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猫疱疹病毒1型基因组短片段的转录分析及一个独特阅读框的插入诱变

Transcriptional analysis of the short segment of the feline herpesvirus type 1 genome and insertional mutagenesis of a unique reading frame.

作者信息

Willemse M J, Strijdveen I G, van Schooneveld S H, van den Berg M C, Sondermeijer P J

机构信息

Virological Research Department, Intervet International B. V., Boxmeer, The Netherlands.

出版信息

Virology. 1995 Apr 20;208(2):704-11. doi: 10.1006/viro.1995.1202.

DOI:10.1006/viro.1995.1202
PMID:7747442
Abstract

Transcription mapping was performed in the short region of the feline herpesvirus type 1 (FHV-1) genome as a first approach to the functional analysis of open reading frames encoding the homologs of the herpes simplex virus type 1 (HSV-1) gD, gl, gE, US9, and probably also US8.5. All reading frames appeared to be transcribed. Transcripts were grouped into two nested RNA sets; namely, the coterminal transcripts of gD and gl and the coterminal transcripts of gE, US8.5, and US9. This situation was similar to that found in the equivalent region of HSV-1. The FHV-1 ORFs US8.5 and US9 have not been described previously. Sequence analysis and comparison of the putative polypeptide encoded by US8.5 revealed that this ORF was unique to FHV-1. However, US8.5 of FHV-1 might be functionally related to its positional homologs in HSV-1 and equine herpesvirus type 1. In all three viruses, US8.5 does not seem to be critical for virus propagation in cell culture. This was shown for FHV-1 by isolating a mutant containing an insertion in US8.5 and comparing its growth properties in cell culture to those of the parent virus G2620. With regard to US9, conscientious amino acid sequence alignment of FHV-1 US9 and homologs in related herpesviruses suggests that this particular protein could contribute to the virus infectivity in vivo. This speculation was based on the highly conserved C-terminus of US9, starting with a characteristic YYSES motif and followed by a nuclear target sequence and a transmembrane region.

摘要

转录图谱绘制是在猫疱疹病毒1型(FHV-1)基因组的短区域进行的,作为对编码单纯疱疹病毒1型(HSV-1)gD、gI、gE、US9以及可能还有US8.5同源物的开放阅读框进行功能分析的第一步。所有阅读框似乎都被转录。转录本被分为两个嵌套的RNA集;即gD和gI的共末端转录本以及gE、US8.5和US9的共末端转录本。这种情况与在HSV-1的等效区域中发现的情况相似。FHV-1的ORF US8.5和US9以前尚未被描述。对US8.5编码的推定多肽进行序列分析和比较发现,这个ORF是FHV-1特有的。然而,FHV-1的US8.5可能在功能上与其在HSV-1和马疱疹病毒1型中的位置同源物相关。在所有这三种病毒中,US8.5似乎对病毒在细胞培养中的增殖并不关键。通过分离出一个在US8.5中含有插入片段的突变体,并将其在细胞培养中的生长特性与亲本病毒G2620的生长特性进行比较,证明了FHV-1的这种情况。关于US9,对FHV-1 US9与相关疱疹病毒中的同源物进行认真的氨基酸序列比对表明,这种特定的蛋白质可能有助于病毒在体内的感染性。这种推测是基于US9高度保守的C末端区域,该区域从一个特征性的YYSES基序开始,接着是一个核靶向序列和一个跨膜区域。

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