[与人类核糖体蛋白L11的mRNA互补的DNA的克隆及一级结构测定]

[Cloning and determination of the primary structure of DNA complementary to the mRNA of human ribosomal protein L11].

作者信息

Mishin V P, Filipenko M L, Muravlev A I, Karpova G G, Mertvetsov N P

出版信息

Bioorg Khim. 1995 Feb;21(2):158-60.

Abstract

A polymerase chain reaction strategy was employed to isolate cDNA encoding L11 human ribosomal protein. Based on the known nucleotide sequence of 5'-region of the ribosomal protein L11 mRNA, we have designed primers and used them in amplification of corresponding sequence of human cDNA from total placenta cDNA. The fragment of RPS26 cDNA was cloned in plasmid vector and sequenced. Sequence analysis showed that there is high homology (88%) between coding regions of RPS26 mRNAs in rat liver and human placenta. The amino acid exchanges were observed at positions: 91 (Asp-->Glu), 217 (Thr-->Ala), 352 (Lys-->Glu).

摘要

采用聚合酶链反应策略分离编码人核糖体蛋白L11的cDNA。基于核糖体蛋白L11 mRNA 5'区域的已知核苷酸序列，我们设计了引物，并用于从胎盘总cDNA中扩增人cDNA的相应序列。将RPS26 cDNA片段克隆到质粒载体中并进行测序。序列分析表明，大鼠肝脏和人胎盘中RPS26 mRNA的编码区之间存在高度同源性（88%）。在以下位置观察到氨基酸交换：91位（天冬氨酸→谷氨酸）、217位（苏氨酸→丙氨酸）、352位（赖氨酸→谷氨酸）。