Alugupalli K R, Kalfas S
Department of Oral Microbiology, Malmö General Hospital, Lund University, Sweden.
APMIS. 1995 Feb;103(2):154-60.
Adhesion of the periodontitis-associated bacteria Actinobacillus actinomycetemcomitans and Prevotella intermedia to monolayers of fibroblasts, HEp-2, KB and HeLa cells was quantified with radiolabeled bacteria. Bacterial adhesion was also examined microscopically with Giemsa-stained non-radioactive preparations. The degree of bacterial adherence was dependent on the growth phase of the bacteria. Strains at the exponential phase adhered to a greater extent than those at the stationary phase of growth. Both human and bovine lactoferrins competitively inhibited the adhesion of A. actinomycetemcomitans and P. intermedia to all tested cell monolayers. The inhibitory effect was dose-dependent in the concentration range 0.5-2500 micrograms/ml and not related to the bacterial growth phase. In the presence of lactoferrin, decreased association of bacteria with the cell monolayers was also found by microscopic examination of the preparations. The present findings indicate that lactoferrin may prevent the establishment of bacteria in periodontal tissues through adhesion-counteracting mechanisms in addition to its bacteriostatic and bactericidal properties.
采用放射性标记细菌的方法，对牙周炎相关细菌伴放线放线杆菌和中间普氏菌与成纤维细胞、人喉表皮癌细胞（HEp-2）、口腔上皮癌细胞（KB）和人宫颈癌细胞（HeLa）单层细胞的黏附情况进行了定量分析。还用吉姆萨染色的非放射性制剂通过显微镜检查细菌黏附情况。细菌的黏附程度取决于细菌的生长阶段。处于指数生长期的菌株比处于稳定生长期的菌株黏附程度更高。人乳铁蛋白和牛乳铁蛋白均能竞争性抑制伴放线放线杆菌和中间普氏菌对所有测试细胞单层的黏附。在0.5 - 2500微克/毫升的浓度范围内，抑制作用呈剂量依赖性，且与细菌生长阶段无关。在乳铁蛋白存在的情况下，通过对制剂的显微镜检查还发现细菌与细胞单层的结合减少。目前的研究结果表明，乳铁蛋白除了具有抑菌和杀菌特性外，还可能通过黏附拮抗机制防止细菌在牙周组织中定植。
