Ogawa M, Miyata T, Nakajima K, Yagyu K, Seike M, Ikenaka K, Yamamoto H, Mikoshiba K
Department of Physiology, Kochi Medical School, Japan.
Neuron. 1995 May;14(5):899-912. doi: 10.1016/0896-6273(95)90329-1.
In the neurological mutant mouse reeler, the histological organization of the neocortex develops abnormally and essentially results in an inversion of the relative positions of the cortical layers. The reeler mutation, therefore, provides an insight into the molecular mechanisms underlying the formation of the cortical layers. We have generated a monoclonal antibody (CR-50) that probes a distinct allelic antigen present in wild-type but not in reeler mutant mice. CR-50 reacted specifically with Cajal-Retzius neurons, one of the first cortical neurons to differentiate in the neocortex, but whose functional role is not known. When dissociated cerebral cortical cells were incubated with CR-50 in reaggregation culture, the genotype-dependent histogenetic assembly of wild-type cortical cells resembled that of reeler mutants. These findings revealed that the selective expression of a distinct molecule on Cajal-Retzius neurons is critical for the normal lamination of cortical neurons in the mammalian neocortex.
在神经学突变小鼠“reeler”中，新皮质的组织学结构发育异常，本质上导致了皮质层相对位置的倒置。因此，“reeler”突变有助于深入了解皮质层形成的分子机制。我们制备了一种单克隆抗体（CR - 50），它能探测野生型小鼠中存在而“reeler”突变小鼠中不存在的一种独特等位基因抗原。CR - 50特异性地与Cajal - Retzius神经元发生反应，Cajal - Retzius神经元是新皮质中最早分化的皮质神经元之一，但其功能作用尚不清楚。当在重聚集培养中用CR - 50孵育解离的大脑皮质细胞时，野生型皮质细胞的基因型依赖性组织发生组装类似于“reeler”突变体。这些发现表明，Cajal - Retzius神经元上一种独特分子的选择性表达对于哺乳动物新皮质中皮质神经元的正常分层至关重要。
