Tamada Y, Kulik E A, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
Biomaterials. 1995 Feb;16(3):259-61. doi: 10.1016/0142-9612(95)92126-q.
A method is proposed for counting the number of adhered platelets based on the determination of lactate dehydrogenase activity in bulk after lysis of adhered platelets. This method was compared with the widely used radioisotope labelling technique. It was concluded that the present lactate dehydrogenase method is effective in counting the adhered platelets, as no significant difference was found between the readings of two methods when commercial polymers and glass were used as samples.
提出了一种基于在裂解粘附血小板后测定总体中乳酸脱氢酶活性来计数粘附血小板数量的方法。该方法与广泛使用的放射性同位素标记技术进行了比较。得出的结论是，当前的乳酸脱氢酶方法在计数粘附血小板方面是有效的，因为当使用商业聚合物和玻璃作为样品时，两种方法的读数之间未发现显著差异。
