Naciri Y, Vigouroux Y, Dallas J, Desmarais E, Delsert C, Bonhomme F
IFREMER Unité de Recherche en Génétique et Ecloserie, La Tremblade, France.
Mol Mar Biol Biotechnol. 1995 Mar;4(1):83-9.
Twelve microsatellites were isolated from a partial genomic library of Ostrea edulis using (GA/TC)n and (AC/GT)n probes and were subsequently sequenced. We estimate that, on average, 12,700 (GA/TC)n and 3900 (AC/GT)n microsatellites could be found in the genome assuming a random distribution. These estimates are high enough for the construction of a saturated genetic map. Primers were designed for three microsatellite loci, and analyses of polymorphism in a wild cohort revealed that one was suitable for population genetics studies (5 alleles), while the other two were highly polymorphic (between 17 and 48 alleles) and thus were more useful for paternity testing. Mendelian inheritance was tested on two full-sib families, and significant distortions of genotypic frequencies were found, although the gametic distributions seemed to be in agreement with Mendelian expectations. We interpret this as evidence for zygotic selection.
使用(GA/TC)n和(AC/GT)n探针从食用牡蛎的部分基因组文库中分离出12个微卫星，随后对其进行测序。我们估计，假设随机分布，基因组中平均可发现12700个(GA/TC)n微卫星和3900个(AC/GT)n微卫星。这些估计值足以构建饱和遗传图谱。为三个微卫星位点设计了引物，对一个野生群体的多态性分析表明，一个适用于群体遗传学研究（5个等位基因），而另外两个具有高度多态性（17至48个等位基因），因此更适用于亲子鉴定。在两个全同胞家系中测试了孟德尔遗传，发现基因型频率存在显著偏差，尽管配子分布似乎符合孟德尔预期。我们将此解释为合子选择的证据。
