García de León F J, Dallas J F, Chatain B, Canonne M, Versini J J, Bonhomme F
Laboratoire Génome et Populations, Université de Montpellier II, France.
Mol Mar Biol Biotechnol. 1995 Mar;4(1):62-8.
The first microsatellite markers in sea bass, Dicentrarchus labrax, are described. Microsatellite-containing clones were isolated from a genomic library, their DNA sequences determined, and polymerase chain reaction primers designed for seven dinucleotide microsatellite loci. All microsatellites were shown to be highly polymorphic: six unrelated individuals displayed 86% observed heterozygosity and an average of 7.6 alleles per locus. Inheritance was shown to be Mendelian by analysis of genotypic ratios in F1 offspring. An analysis of 97 mass-reared offspring of a complete diallel cross between three males and three females showed that the parentage of all individuals could be determined unambiguously after scoring one locus in all individuals, followed by scoring a second locus in 24 individuals. The results suggest that microsatellites will be valuable tools in studies of sea bass for genetic tagging, marker-assisted selection programs, and identification of natural stocks.
描述了鲈鱼（Dicentrarchus labrax）中的首批微卫星标记。从基因组文库中分离出含微卫星的克隆，测定其DNA序列，并为7个二核苷酸微卫星位点设计了聚合酶链反应引物。所有微卫星均显示出高度多态性：6个无亲缘关系的个体表现出86%的观察杂合度，每个位点平均有7.6个等位基因。通过分析F1后代的基因型比例，证明遗传符合孟德尔遗传规律。对3只雄性和3只雌性之间完全双列杂交的97只大规模养殖后代进行分析表明，在对所有个体的一个位点进行评分后，再对24个个体的第二个位点进行评分，就可以明确确定所有个体的亲本。结果表明，微卫星将成为鲈鱼遗传标记、标记辅助选择计划和天然种群鉴定研究中的有价值工具。
