去除细胞外钠离子对体外大鼠下颌下腺钾离子通透性胆碱能调节的影响。

The effect of removing external sodium upon the cholinergic regulation of potassium permeability in the rat submandibular gland in vitro.

作者信息

Pediani J D, McEwan P E, Elder H Y, Wilson S M

机构信息

Institute of Physiology, University of Glasgow, U.K.

出版信息

Comp Biochem Physiol Pharmacol Toxicol Endocrinol. 1994 Feb;107(2):283-8. doi: 10.1016/1367-8280(94)90052-3.

DOI:10.1016/1367-8280(94)90052-3

PMID:7749595

Abstract

Fragments of rat submandibular gland were loaded with 86Rb+ and superfused so that the rate of 86Rb(+)-efflux could be quantified as an indicator of potassium permeability. Acetylcholine evoked an increase in permeability consisting of a transient, calcium-independent response and a sustained, calcium-dependent. Total removal of external sodium significantly inhibited both phases of this response. The results thus confirm that the cholinergic regulation of potassium permeability is compromised by removal of external sodium but do not support the view that this is due, exclusively, to an effect on calcium influx.

摘要

将大鼠颌下腺组织块用⁸⁶Rb⁺加载并进行灌流，以便将⁸⁶Rb⁺外流速率定量作为钾通透性的指标。乙酰胆碱引起通透性增加，包括一个短暂的、不依赖钙的反应和一个持续的、依赖钙的反应。完全去除细胞外钠显著抑制了该反应的两个阶段。因此，结果证实细胞外钠的去除损害了胆碱能对钾通透性的调节，但不支持这完全是由于对钙内流的影响这一观点。