Pediani J D, Wilson S M
Institute of Physiology, University of Glasgow, Scotland, UK.
Experientia. 1995 Feb 15;51(2):110-2. doi: 10.1007/BF01929350.
Acetylcholine releases calcium from cytoplasmic stores and permits an influx of calcium in salivary acinar cells. The resultant rise in [Ca2+]i causes an increase in potassium permeability which is an important part of the secretory response. We have investigated the effects of 12-O-tetradecanoyl phorbol-13-acetate, a potent activator of protein kinase C, upon this regulation of potassium permeability in superfused pieces of rat submandibular salivary gland. This compound inhibited the initial [Ca2+]O-independent component of the response of acetylcholine but had no effect upon the subsequent [Ca2+]O-dependent phase. This compound does not, therefore, appear to inhibit receptor-regulated calcium influx.
乙酰胆碱从细胞质储存库释放钙，并使钙流入唾液腺泡细胞。由此导致的细胞内钙离子浓度（[Ca2+]i）升高会引起钾离子通透性增加，这是分泌反应的一个重要部分。我们研究了蛋白激酶C的强效激活剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯对大鼠下颌下唾液腺灌流组织中钾离子通透性这种调节作用的影响。该化合物抑制了乙酰胆碱反应中最初不依赖细胞外钙离子浓度（[Ca2+]O）的成分，但对随后依赖[Ca2+]O的阶段没有影响。因此，该化合物似乎并不抑制受体调节的钙内流。
