Egaas E, Falls J G, Dauterman W C
Norwegian Plant Protection Institute, As.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1995 Jan;110(1):35-40. doi: 10.1016/0742-8413(94)00079-p.
The hepatic cytosolic glutathione S-transferase (GST) activity in four strains of the mouse and one strain of the rat was studied with the substrates 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB), ethachrynic acid (ETHA), cumene hydroperoxide (CU) and atrazine as the in vitro substrates. In the mouse, significant gender, strain and age-related differences in the GST activity towards CDNB and atrazine were found between adolescent and sexually mature males and females of the CD-1, C57BL/6, DBA/2 and Swiss-Webster strains, and the differences were larger with atrazine as the substrate. With DCNB and CU a similar tendency was observed, however not significant for all strains. The GST activity towards ETHA was also gender and strain specific, but revealed no age-related differences. The herbicide atrazine seems to be a useful substrate in the study of strain and age-related differences in the mouse GST class Pi.
以1-氯-2,4-二硝基苯（CDNB）、1,2-二氯-4-硝基苯（DCNB）、依他尼酸（ETHA）、氢过氧化异丙苯（CU）和莠去津作为体外底物，研究了四种品系小鼠和一种品系大鼠的肝细胞质谷胱甘肽S-转移酶（GST）活性。在小鼠中，发现CD-1、C57BL/6、DBA/2和瑞士 Webster品系的青春期和性成熟雄性及雌性之间，GST对CDNB和莠去津的活性存在显著的性别、品系和年龄相关差异，以莠去津为底物时差异更大。对于DCNB和CU也观察到类似趋势，但并非所有品系都显著。GST对ETHA的活性也具有性别和品系特异性，但未显示出年龄相关差异。除草剂莠去津似乎是研究小鼠GST π类品系和年龄相关差异的有用底物。
