Wieser J, Lee B N, Fondon J w, Adams T H
Department of Biology, Texas A & M University, College Station 77843, USA.
Curr Genet. 1994 Dec;27(1):62-9. doi: 10.1007/BF00326580.
Conidiation in the filamentous ascomycete Aspergillus nidulans requires activation of brlA, a well-characterized transcriptional regulator of genes that are induced specifically during asexual development. We have isolated and characterized developmental mutations in six loci, designated fluG, flbA, flbB, flbC, flbD, and flbE, that result in defective development and reduced brlA expression. These mutants grow indeterminately to produce masses of aerial hyphae resulting in the formation of cotton-like colonies with a "fluffy" morphology. The results of growth and epistasis tests involving all pairwise combinations of fluffy mutations indicate complex hierarchical relationships among these loci. We discuss these genetic interactions and propose that there are multiple mechanisms for activating brlA.
丝状子囊菌构巢曲霉的分生孢子形成需要brlA的激活，brlA是一种已被充分表征的转录调节因子，可调节在无性发育过程中特异性诱导的基因。我们已经分离并鉴定了六个位点的发育突变，分别命名为fluG、flbA、flbB、flbC、flbD和flbE，这些突变导致发育缺陷和brlA表达降低。这些突变体无限生长，产生大量气生菌丝，形成具有“蓬松”形态的棉絮状菌落。涉及所有蓬松突变两两组合的生长和上位性测试结果表明，这些位点之间存在复杂的层次关系。我们讨论了这些遗传相互作用，并提出存在多种激活brlA的机制。
