Price E A, Coombe D R, Murray J C
University of Nottingham Laboratory of Molecular Oncology, City Hospital, UK.
Clin Exp Metastasis. 1995 May;13(3):155-64. doi: 10.1007/BF00132203.
A static adhesion assay employing 6-carboxy-3',6'-diacetylfluorescein (6-CFDA) as a fluorescent marker has been developed to study the interactions of tumour cell lines with endothelial monolayers. This assay allows simple, safe quantification of cell-cell adhesion using living cells. It has been used to demonstrate that the integrin adhesion molecule VLA-4 mediates the attachment of RPMI-7951 melanoma cells to human umbilical vein endothelial cells (HUVEC) which have been activated by TNF alpha. In addition, MDA-MB-231 breast adenocarcinoma cells display greater adhesion to microvessel endothelial cells than to large vessel endothelial cells.
一种采用6-羧基-3',6'-二乙酰荧光素（6-CFDA）作为荧光标记物的静态黏附试验已被开发出来，用于研究肿瘤细胞系与内皮细胞单层之间的相互作用。该试验能够使用活细胞对细胞间黏附进行简单、安全的定量分析。它已被用于证明整合素黏附分子VLA-4介导RPMI-7951黑色素瘤细胞与经肿瘤坏死因子α激活的人脐静脉内皮细胞（HUVEC）的黏附。此外，MDA-MB-231乳腺腺癌细胞对微血管内皮细胞的黏附力比对大血管内皮细胞的黏附力更强。
