Maehara M, Yagita M, Isobe Y, Hoshino T, Nakagawara G
First Department of Surgery, Fukui Medical School, Japan.
Int J Cancer. 1993 May 8;54(2):296-301. doi: 10.1002/ijc.2910540222.
Dimethyl sulfoxide (DMSO) exerts a number of biological effects including the promotion of cell differentiation in cultured cells. In this study, we examined the effect of DMSO on the adhesion of tumor cells to endothelial cells. In vitro treatment of human gastric adenocarcinoma (NUGC4) cells with DMSO resulted in increased adhesion to interleukin-I (IL-I)-activated human endothelial cells compared with DMSO-untreated NUGC4 cells. In flow cytometry, treating NUGC4 cells with DMSO enhanced the expression of sialyl Lewis x (sialyl Le(x)) and sialyl dimeric Le(x) antigens on their surface. Also, the binding of Limulus polyphemus agglutinin (LPA), which specifically binds to cell-surface sialic acids, was increased by DMSO. The adhesion of DMSO-treated NUGC4 cells to activated endothelial cells was blocked by neuraminidase pre-treatment of tumor cells or by antibody against either endothelial leukocyte adhesion molecule-I (ELAM-I) or sialyl Le(x). Thus, it is suggested that enhanced adhesion following DMSO treatment is mediated by the interaction of sialyl Le(x) expressed on NUGC4 cells with ELAM-I of endothelial cells. The modulation of sialyl Le(x) antigen by DMSO provides a useful system for studying the regulatory mechanism of Lewis-related carbohydrate antigens and also for understanding the metastatic properties of cancer cells.
二甲基亚砜（DMSO）具有多种生物学效应，包括促进培养细胞的分化。在本研究中，我们检测了DMSO对肿瘤细胞与内皮细胞黏附的影响。与未用DMSO处理的NUGC4细胞相比，用DMSO体外处理人胃腺癌（NUGC4）细胞后，其与白细胞介素 -I（IL-I）激活的人内皮细胞的黏附增加。在流式细胞术中，用DMSO处理NUGC4细胞可增强其表面唾液酸化路易斯x（唾液酸化Le(x)）和唾液酸化二聚体Le(x)抗原的表达。此外，DMSO可增加鲎试剂凝集素（LPA）（其特异性结合细胞表面唾液酸）的结合。用神经氨酸酶预处理肿瘤细胞或用抗内皮细胞白细胞黏附分子-I（ELAM-I）或唾液酸化Le(x)的抗体可阻断经DMSO处理的NUGC4细胞与活化内皮细胞的黏附。因此，提示DMSO处理后黏附增强是由NUGC4细胞上表达的唾液酸化Le(x)与内皮细胞的ELAM-I相互作用介导的。DMSO对唾液酸化Le(x)抗原的调节为研究路易斯相关碳水化合物抗原的调控机制以及理解癌细胞的转移特性提供了一个有用的系统。
