Hirayma J, Kamo N, Imamoto Y, Shichida Y, Yoshizawa T
Department of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
FEBS Lett. 1995 May 8;364(2):168-70. doi: 10.1016/0014-5793(95)00381-i.
The reconstitution of pharaonis phoborhodopsin was performed by incubation of its opsin with 13-cis-retinal. Spectrum change was very slow, and two phases of the change were observed: the first and second phases are due to the transient formation of 13-cis pigment and spontaneous isomerization to all-trans-retinal, respectively. Slow binding supports an idea that the retinal binding pocket of ppR is highly restricted. Being bent in the configuration, 13-cis-retinal cannot be accommodated in the pocket due to the steric hindrance. This is a possible reason for the lack of light-dark adaptation.
通过将法老视紫红质的视蛋白与13-顺式视黄醛一起孵育来进行其重组。光谱变化非常缓慢，并且观察到变化的两个阶段：第一阶段和第二阶段分别归因于13-顺式色素的瞬时形成和向全反式视黄醛的自发异构化。缓慢结合支持了一个观点，即ppR的视黄醛结合口袋高度受限。由于空间位阻，呈弯曲构型的13-顺式视黄醛无法容纳在该口袋中。这可能是缺乏明暗适应的一个原因。
