Jacobsen G K, Povlsen C O, Rygaard J
Tokai J Exp Clin Med. 1983 Dec;8(5-6):513-27.
The effects of neonatal murine thymus grafts implanted in nude mice previously transplanted with three different human malignant tumours were studied. Reconstitution resulted in tumour rejection, which started 2-3 weeks after thymus implantation and was complete after 3-6 weeks. The rejection process showed a characteristic histologic picture with 3 phases, an early, an intermediate, and a late phase, which were similar in the 3 tumour types examined. Histological examination of lymphoid tissues of successfully thymus grafted mice showed reconstitution of varying degrees. Numbers of plaque forming cells (PFC) of the spleen of tumour transplanted, thymus grafted mice were equal to or higher than the numbers of PFC in normal BALB/c controls. Mice, thymus grafted only always showed intermediate PFC-values. Responses to the T-cell mitogen, phytohaemagglutinin-P (PHA) were only partially reconstituted, whether the nude mice were tumour transplanted or not. In addition tumour rejection in tumour-bearing nude mice treated with T-cells only, was studied. The rejection process was similar to that observed when a whole thymus gland was implanted. Finally, implantation of embryonic bovine thymus grafts was performed in tumour transplanted nude mice. When these grafts were accepted, tumours were also rejected. Possible mechanisms of reconstitution and the reasons for the varying results are discussed.
研究了将新生小鼠胸腺移植到先前移植了三种不同人类恶性肿瘤的裸鼠体内的效果。重建导致肿瘤排斥反应，该反应在胸腺植入后2 - 3周开始，3 - 6周后完全完成。排斥过程呈现出具有三个阶段的特征性组织学图像，即早期、中期和晚期，在所检查的三种肿瘤类型中相似。对成功进行胸腺移植的小鼠的淋巴组织进行组织学检查显示出不同程度的重建。肿瘤移植且胸腺移植小鼠脾脏中的空斑形成细胞（PFC）数量等于或高于正常BALB/c对照中的PFC数量。仅进行胸腺移植的小鼠总是显示出中等的PFC值。无论裸鼠是否进行肿瘤移植，对T细胞有丝分裂原植物血凝素-P（PHA）的反应仅部分重建。此外，还研究了仅用T细胞治疗的荷瘤裸鼠中的肿瘤排斥反应。排斥过程与植入整个胸腺时观察到的相似。最后，在肿瘤移植的裸鼠中进行胚胎牛胸腺移植。当这些移植被接受时，肿瘤也被排斥。讨论了重建的可能机制以及结果差异的原因。
