Salerno A, Dieli F, Sireci G, Bellavia A, Asherson G L
Institute of General Pathology, University of Palermo, Italy.
Immunology. 1995 Mar;84(3):404-9.
This study demonstrates an essential role for interleukin-4 (IL-4) in the delayed hypersensitivity reaction, as illustrated by contact sensitivity (CS) to trinitrochlorobenzene (TNCB). Injection of mice with monoclonal antibody to IL-4, but not with control antibody, reduced CS after active immunization by 75%, as judged by ear swelling. The histological alterations of CS were also reduced. IL-4 was essential to the effector stage, as inhibition of its production or action blocked the passive transfer of CS. In particular, treatment of immune lymph node cells with antisense oligonucleotide to IL-4 inhibited the systemic transfer of CS. Transfer was also inhibited by monoclonal antibody to IL-4 given to the recipient. The present results indicate that IL-4 is an essential cytokine at the effector stage of the CS reaction.
本研究证明了白细胞介素-4（IL-4）在迟发型超敏反应中的重要作用，如对三硝基氯苯（TNCB）的接触性敏感（CS）所示。通过耳部肿胀判断，给小鼠注射抗IL-4单克隆抗体而非对照抗体，可使主动免疫后的CS降低75%。CS的组织学改变也有所减轻。IL-4对效应阶段至关重要，因为抑制其产生或作用会阻断CS的被动转移。特别是，用抗IL-4反义寡核苷酸处理免疫淋巴结细胞可抑制CS的全身转移。给受体注射抗IL-4单克隆抗体也会抑制转移。目前的结果表明，IL-4是CS反应效应阶段的一种重要细胞因子。
