Dieli F, Asherson G L, Sireci G, Dominici R, Gervasi F, Vendetti S, Colizzi V, Salerno A
Institute of General Pathology, University of Palermo, Italy.
Eur J Immunol. 1997 Jan;27(1):206-14. doi: 10.1002/eji.1830270131.
Ptak and Askenase showed that both alphabeta and gammadelta cells are required for transfer of contact sensitivity (CS). This study confirms that day 4 immune cells depleted of gammadelta cells fail to transfer CS to trinitrochlorobenzene (TNP-Cl) systemically and demonstrates that administration of anti-gammadelta monoclonal antibodies (mAb) in vivo abolishes the CS reaction. Moreover, gammadelta cells accumulate at the antigen challenge site: these cells have the unusual phenotype CD8alpha+, CD8beta-, IL-4 R+ which we suggest is due to their state of activation. Following immunization with contact sensitizer on the skin, the absolute number of gammadelta cells increases in the regional lymph nodes with a peak at 4 days. Of the gammadelta cells, 80 %, both in the lymph nodes of TNP-Cl-immune mice and accumulating at the antigen challenge site are Vgamma3+. The gammadelta cells expressing Vgamma3, which is characteristic of dendritic epithelial T cells (DETC), obtained 4 days after sensitization, proliferate in response to interleukin (IL)-7, but only poorly to IL-2 and IL-4. They also respond to concanavalin A and immobilized anti-gammadelta mAb, but not to haptens or heat-shocked syngeneic spleen cells. Furthermore, injection of mice with mAb to IL-7 inhibits accumulation of Vgamma3+ cells both in the lymph nodes after skin sensitization and at the antigen-challenge site. Altogether, these results strongly support the view that DETC are related to, or the original source of, the gammadelta cells found in the lymph node after skin sensitization and at the site of challenge, and that IL-7 is implicated in these phenomena.
普塔克和阿斯凯纳斯指出，αβ细胞和γδ细胞都是接触敏感性（CS）传递所必需的。本研究证实，缺失γδ细胞的第4天免疫细胞无法将CS全身性地传递至三硝基氯苯（TNP-Cl）系统，并证明体内给予抗γδ单克隆抗体（mAb）可消除CS反应。此外，γδ细胞在抗原攻击部位聚集：这些细胞具有不寻常的表型CD8α +、CD8β -、IL-4R +，我们认为这是由于它们的激活状态所致。在皮肤用接触致敏剂免疫后，γδ细胞的绝对数量在局部淋巴结中增加，在第4天达到峰值。在γδ细胞中，TNP-Cl免疫小鼠淋巴结中以及在抗原攻击部位聚集的细胞中，80%是Vγ3 +。致敏后4天获得的表达Vγ3的γδ细胞，其具有树突状上皮T细胞（DETC）的特征，对白细胞介素（IL）-7有增殖反应，但对IL-2和IL-4的反应较差。它们也对伴刀豆球蛋白A和固定化抗γδ mAb有反应，但对半抗原或热休克同基因脾细胞无反应。此外，给小鼠注射抗IL-7 mAb可抑制皮肤致敏后淋巴结中以及抗原攻击部位Vγ3 +细胞的聚集。总之，这些结果有力地支持了以下观点：DETC与皮肤致敏后淋巴结以及攻击部位发现的γδ细胞相关或为其原始来源，并且IL-7与这些现象有关。
