Kaufman L, Sekhon A S, Moledina N, Jalbert M, Pappagianis D
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1995 Mar;33(3):618-9. doi: 10.1128/jcm.33.3.618-619.1995.
A total of 409 serum and cerebrospinal fluid specimens from human subjects with proven coccidioidomycosis, with other infections, or with no apparent illness were tested for antibodies to Coccidioides immitis by the Premier EIA (Meridian Diagnostics, Inc., Cincinnati, Ohio), which tests for immunoglobulin G (IgG) and IgM responses to coccidioidal antigens, and by the conventional complement fixation (CF) or immunodiffusion (ID) assays for antibodies corresponding to those detected by the tube precipitin (TP) or CF tests. Of the 409 specimens, 47 were from persons with confirmed coccidioidomycosis and all were positive for C. immitis antibodies in IDCF tests and enzyme immunoassays (EIAs) for both IgG and IgM. The EIA for detecting both IgG and IgM antibodies proved to be sensitive for detecting coccidioidomycosis case sera positive by the IDCF, IDTP, and CF tests. Maximal sensitivity for diagnosing coccidioidomycosis is dependent upon detection of both IgG and IgM antibodies in the EIA. The EIA, however, was not absolutely specific, since some sera from patients with confirmed blastomycosis and some from patients with noncoccidioidal disease produced false-positive reactions.
采用Premier EIA（Meridian诊断公司，俄亥俄州辛辛那提）对409份来自确诊球孢子菌病、患有其他感染或无明显疾病的人类受试者的血清和脑脊液标本进行检测，该方法用于检测针对球孢子菌抗原的免疫球蛋白G（IgG）和IgM反应，同时采用传统的补体结合（CF）或免疫扩散（ID）试验检测与试管沉淀素（TP）或CF试验检测到的抗体相对应的抗体。在这409份标本中，47份来自确诊球孢子菌病的患者，所有这些患者的标本在IDCF试验以及针对IgG和IgM的酶免疫测定（EIA）中，球孢子菌抗体均呈阳性。检测IgG和IgM抗体的EIA被证明对检测经IDCF、IDTP和CF试验呈阳性的球孢子菌病病例血清具有敏感性。诊断球孢子菌病的最大敏感性取决于在EIA中同时检测到IgG和IgM抗体。然而，EIA并非绝对特异，因为一些来自确诊芽生菌病患者的血清以及一些来自非球孢子菌病患者的血清会产生假阳性反应。
