来自艾滋病患者且显示不同氟康唑耐药谱的念珠菌分离株的分子流行病学。
Molecular epidemiology of Candida isolates from AIDS patients showing different fluconazole resistance profiles.
作者信息
Lischewski A, Ruhnke M, Tennagen I, Schönian G, Morschhäuser J, Hacker J
机构信息
Zentrum für Infektionsforschung, Universität Würzburg, Germany.
出版信息
J Clin Microbiol. 1995 Mar;33(3):769-71. doi: 10.1128/jcm.33.3.769-771.1995.
Abstract
Thirty Candida isolates obtained from the oropharynxes of three AIDS patients were genotypically characterized. In vitro fluconazole MIC determination revealed increasing fluconazole resistances during treatment, thereby confirming the in vivo situation. Pulsed-field gel electrophoresis karyotyping, randomly amplified polymorphic DNA analysis, and hybridizations with Candida albicans repetitive element 2 were used to determine possible genotypic changes. The isolates from two patients showed genetic homogeneity, suggesting the selection for resistant variants. One patient experienced a strain switch to Candida krusei. Horizontal spread of identical strains between the patients could be excluded. However, the molecular methods used might not be sufficient to detect the underlying genetic basis of resistance to fluconazole.
摘要
对从三名艾滋病患者口咽部分离出的30株念珠菌进行了基因分型鉴定。体外氟康唑最低抑菌浓度测定显示，治疗期间氟康唑耐药性不断增加，从而证实了体内情况。采用脉冲场凝胶电泳核型分析、随机扩增多态性DNA分析以及与白色念珠菌重复元件2杂交的方法来确定可能的基因型变化。两名患者的分离株显示出基因同质性，提示存在耐药变异株的选择。一名患者发生了菌株转换为克柔念珠菌。可以排除患者之间相同菌株的水平传播。然而，所使用的分子方法可能不足以检测氟康唑耐药性的潜在遗传基础。
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