Jain P, Khan Z K, Bhattacharya E, Ranade S A
Medical Mycology Division, Central Drug Research Institute, P.O. Box 173, 226 001, Lucknow, India.
Diagn Microbiol Infect Dis. 2001 Nov;41(3):113-9. doi: 10.1016/s0732-8893(01)00292-9.
Random amplified polymorphic DNA analysis was used to detect genotype relatedness among clinical fluconazole-resistant and -sensitive strains of Candida albicans recovered from twenty HIV-infected patients having oropharyngeal candidiasis. Sensitive strains were obtained from a local hospital and were from patients that had not been treated with azole drugs while resistant strains were recovered from patients in different parts of Europe and their resistance was a consequence of drug-treatment given to the patients. On amplification with different arbitrary sequence decamer primers, the results demonstrated a homogeneous banding pattern for all sensitive strains that was distinct from that obtained in case of the resistant strains. The DNA profiles of strains were thus broadly clustered into two major groups of resistant and sensitive strains. The RAPD technique may be useful in differentiating fluconazole-resistant strains from the -sensitive ones for early identification of resistant isolates from AIDS patients.
随机扩增多态性DNA分析被用于检测从20例患有口腔念珠菌病的HIV感染患者中分离出的白色念珠菌临床氟康唑耐药和敏感菌株之间的基因型相关性。敏感菌株来自当地一家医院，取自未接受过唑类药物治疗的患者，而耐药菌株则从欧洲不同地区的患者中分离得到，其耐药性是患者接受药物治疗的结果。用不同的任意序列十聚体引物进行扩增后，结果显示所有敏感菌株具有相同的条带模式，这与耐药菌株的条带模式明显不同。因此，菌株的DNA图谱大致分为耐药和敏感两大主要类别。RAPD技术可能有助于区分氟康唑耐药菌株和敏感菌株，以便早期从艾滋病患者中鉴定出耐药菌株。
