Tsukamoto T, Kawai T
Osaka Prefectural Institute of Public Health.
Kansenshogaku Zasshi. 1995 Jan;69(1):85-90. doi: 10.11150/kansenshogakuzasshi1970.69.85.
We screened for the presence of Escherichia coli eae gene, one of the pathogenic factors of enteropathogenic E. coli (EPEC), by using the PCR method and compared the results with those from localized adherence to HeLa cells, presence of the EAF plasmid and the serotyping. One thousand one hundred and four E. coli strains isolated from diarrhea were examined, 144 (13.0%) possessed the eag gene. Of these eae positive strains, 93 (8.4%) exhibited localized adherence to HeLa cells and carried the EAF plasmid. And of these, 79 (7.2%) belonged to EPEC serotypes and 14 (1.3%) belonged to non-EPEC serotypes. Five (0.5%) with weak adherence, and 46 (4.2%) with no adherence were EAF plasmid negative. Predominant serotypes of EPEC were O55:H-, O86:H34, O111:H2, O119:H6, O128:H2 and main serotype of non-EPEC was O88:H25. Predominant serotypes of E. coli with no adherence to HeLa cells were O26:H-, O55:H-, O101:H-. EPEC and non-EPEC with localized adherence to HeLa cells were associated with diarrhea. Some of the eae positive strains with no adherence to HeLa cells, may have lost the EAF plasmid during storage. Though several variants of eae gene exist, it is necessary to confirm whether the strains possessing the eae gene without localized adherence to HeLa cells can cause diarrhea.
我们采用聚合酶链反应（PCR）方法筛查肠致病性大肠杆菌（EPEC）致病因子之一的大肠杆菌eae基因的存在情况，并将结果与HeLa细胞局部黏附试验、EAF质粒的存在情况以及血清分型的结果进行比较。对从腹泻患者中分离出的1104株大肠杆菌进行检测，144株（13.0%）携带eae基因。在这些eae阳性菌株中，93株（8.4%）表现出对HeLa细胞的局部黏附并携带EAF质粒。其中，79株（7.2%）属于EPEC血清型，14株（1.3%）属于非EPEC血清型。5株（0.5%）黏附较弱，46株（4.2%）无黏附的菌株EAF质粒阴性。EPEC的主要血清型为O55:H-、O86:H34、O111:H2、O119:H6、O128:H2，非EPEC的主要血清型为O88:H25。不黏附HeLa细胞的大肠杆菌主要血清型为O26:H-、O55:H-、O101:H-。对HeLa细胞有局部黏附的EPEC和非EPEC与腹泻有关。一些不黏附HeLa细胞的eae阳性菌株可能在保存过程中丢失了EAF质粒。尽管存在几种eae基因变体，但有必要确认那些携带eae基因却不黏附HeLa细胞的菌株是否会引起腹泻。
