Tanaka D, Gyobu Y, Kodama H
Toyama Institute of Health.
Kansenshogaku Zasshi. 1995 Apr;69(4):455-60. doi: 10.11150/kansenshogakuzasshi1970.69.455.
Restriction endonuclease (Sma I) digestion patterns of chromosomal DNAs from 459 group B streptococci (GBS) isolated in two hospitals (C and S) in Toyama Prefecture were analysed by pulsed-field gel electrophoresis (PFGE). Results were summarized as follows. 1) One hundred thirty eight isolates of GBS serotype Ia, 174 of serotype III, 102 of serotype JM-9 and 45 of serotype NT-6 were further divided into 56, 41, 36 and 19 PFGE types, respectively. 2) Appreciable differences in the distribution of PFGE types were not observed between C and S hospital isolates, nor among specimens from which GBS strains were isolated. 3) Long-term inspection of 5 patients, from whom the same serotype strains were repeatedly isolated, revealed that some changes in PFGE types were observed in 2 patients, but not in the other 3.
采用脉冲场凝胶电泳（PFGE）分析了从富山县两家医院（C医院和S医院）分离出的459株B组链球菌（GBS）染色体DNA的限制性内切酶（Sma I）消化模式。结果总结如下：1）138株GBS Ia血清型菌株、174株III血清型菌株、102株JM-9血清型菌株和45株NT-6血清型菌株分别进一步分为56种、41种、36种和19种PFGE型。2）在C医院和S医院分离的菌株之间，以及分离出GBS菌株的标本之间，未观察到PFGE型分布的明显差异。3）对5例反复分离出相同血清型菌株的患者进行长期观察发现，2例患者的PFGE型出现了一些变化，而其他3例未出现变化。
