Tanaka D, Gyobu Y, Kodama H
Toyama Institute of Health.
Kansenshogaku Zasshi. 1996 Mar;70(3):283-6. doi: 10.11150/kansenshogakuzasshi1970.70.283.
Restriction endonuclease (Sma I) digestion patterns of chromosomal DNAs of T types 1 and 3 (T1 and T3) group A streptococci (GAS), isolated at two hospitals in Toyama Prefecture from 1983 to 1994, were analysed by pulsed-filed gel electrophoresis (PFGE). One hundred twenty seven isolated of T1 GAS were further divided into 5 PFGE types, which were provisionally designated as T1-1 approximately T1-5. Two epidemics of T1 GAS were observed during the 1983 approximately 1994 period, the first of which (1887 approximately 1988) seemed to be caused by T1-2, and the second (1991 approximately 1993) by T1-5. Some differences in biological characteristics between the two PFGE type strains were also observed. On the other hand, eighty one T3 GAS strains were divided into only two PFGE types, provisionally designated as T3-1 and T3-2, most of which belonged to T3-2. Naturally, both epidemics by T3 GAS, first from 1985 to 1986 and second from 1993 to 1994, seemed to be caused by the same PFGE type, T3-2. No differences in biological characteristics were observed between T3-1 and T3-2 strains.
采用脉冲场凝胶电泳（PFGE）分析了1983年至1994年期间从富山县两家医院分离出的A组1型和3型（T1和T3）链球菌（GAS）染色体DNA的限制性内切酶（Sma I）消化模式。127株T1 GAS进一步分为5种PFGE型，暂定为T1-1至T1-5。在1983年至1994年期间观察到两起T1 GAS流行，第一起（1887年至1988年）似乎由T1-2引起，第二起（1991年至1993年）由T1-5引起。还观察到两种PFGE型菌株在生物学特性上的一些差异。另一方面，81株T3 GAS菌株仅分为两种PFGE型，暂定为T3-1和T3-2，其中大多数属于T3-2。自然地，T3 GAS的两起流行，第一起从1985年至1986年，第二起从1993年至1994年，似乎由同一PFGE型T3-2引起。未观察到T3-1和T3-2菌株在生物学特性上的差异。
