Ellis S, Kotiw M, Garland S M
Department of Medicine, University of Melbourne, Geelong Hospital, Victoria, Australia.
J Hosp Infect. 1996 Aug;33(4):279-87. doi: 10.1016/s0195-6701(96)90014-6.
This study examined isolates of group B streptococci (GBS) by latex seroagglutination and restriction endonuclease analysis (REA). REA analysis was performed with high- and low-frequency cutting enzymes, and fragments were resolved by linear and pulsed-field gel electrophoresis (PFGE), respectively. GBS isolates were derived from a regional acute-care hospital and a tertiary referral obstetric hospital. The most common serotype isolated at the general hospital was serotype V (42%). A high incidence of this serotype V was associated with wound infections, but there was no predominant REA profile. Serotype III was the most common from the obstetric hospital (41%). The majority of these isolates were associated with neonatal GBS infection and there was a predominant restriction pattern. Analysis of restriction patterns of GBS isolated from individual mother/ baby pairs revealed common patterns. These data extend the evidence for vertical transmission of GBS and clones of serotype III GBS with increased pathogenic potential.
本研究通过乳胶血清凝集试验和限制性内切酶分析（REA）对B族链球菌（GBS）分离株进行了检测。REA分析使用了高频和低频切割酶，片段分别通过线性和脉冲场凝胶电泳（PFGE）进行分离。GBS分离株来自一家地区性急症护理医院和一家三级转诊产科医院。综合医院分离出的最常见血清型是V型（42%）。V型血清型的高发病率与伤口感染有关，但没有主要的REA图谱。III型血清型是产科医院最常见的（41%）。这些分离株中的大多数与新生儿GBS感染有关，并且存在一种主要的限制性模式。对从母婴个体对中分离出的GBS的限制性模式分析显示出常见模式。这些数据扩展了GBS垂直传播以及具有增强致病潜力的III型GBS克隆体的证据。
