Malemud C J, Goldberg V M
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4946, USA.
J Rheumatol Suppl. 1995 Feb;43:91-3.
Proteoglycans synthesized by human cartilage were studied in explants and in chondrocyte cultures. The D1 fraction proteoglycans radiolabelled with 35SO4 were analyzed by 3-16% sodium dodecyl sulfate polyacrylamide gel electrophoresis or on 3-5% acrylamide gels. After 24 h in culture, osteoarthritic and age matched nonarthritic cartilage synthesized one aggrecan core protein (LI) whose apparent size after chondroitinase ABC/keratanase digestion was approximately 520 kDa. Three proteoglycan core proteins were found in the D1 fraction of the medium compartment of nonarthritic chondrocytes, whose apparent sizes were approximately 520 kDa (LI), approximately 390 kDa (LII), and approximately 480 kDa (LIII). The LII and LIII core proteins were routinely absent from osteoarthritic chondrocytes. Chondrocytes from a 28-year-old patient with osteoarthritis secondary to congenital hip dysplasia synthesized principally the LI proteoglycan core protein.
在软骨外植体和软骨细胞培养物中研究了人软骨合成的蛋白聚糖。用35SO4放射性标记的D1级分蛋白聚糖通过3-16%十二烷基硫酸钠聚丙烯酰胺凝胶电泳或在3-5%丙烯酰胺凝胶上进行分析。培养24小时后，骨关节炎和年龄匹配的非骨关节炎软骨合成了一种聚集蛋白聚糖核心蛋白（LI），经软骨素酶ABC/角质酶消化后其表观大小约为520 kDa。在非骨关节炎软骨细胞培养基隔室的D1级分中发现了三种蛋白聚糖核心蛋白，其表观大小分别约为520 kDa（LI）、约390 kDa（LII）和约480 kDa（LIII）。骨关节炎软骨细胞中通常不存在LII和LIII核心蛋白。一名因先天性髋关节发育不良继发骨关节炎的28岁患者的软骨细胞主要合成LI蛋白聚糖核心蛋白。
