Poole A R, Rosenberg L C, Reiner A, Ionescu M, Bogoch E, Roughley P J
Joint Diseases Laboratory, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada.
J Orthop Res. 1996 Sep;14(5):681-9. doi: 10.1002/jor.1100140502.
The study was designed to determine the contents and distributions of the proteoglycans decorin and biglycan in adult human femoral condylar cartilage and whether these may change in osteoarthritis. New radioimmunoassays were established using peptides representing the amino-terminal 21 amino acid sequence of each proteoglycan (to which a tyrosine was added for radioiodination) and antibodies in a rabbit antiserum raised to both these molecules. Cartilage was extracted with 4 M guanidine HCl to determine total content, and extracts were analyzed by chromatography to determine molecular sizes. Frozen sections were cut parallel to the articular surface and were extracted to determine distribution within the tissue. Gel chromatography on Sepharose CL-2B under dissociative conditions revealed molecules with a partition coefficient of 0.7-0.75 in both normal and osteoarthritic cartilage. In normal adult cartilage, the mean contents of the core proteins of biglycan and decorin were calculated to be approximately 0.34 and 0.48 mg per gram wet weight, respectively. These represented molar contents similar to that of aggrecan. In osteoarthritic cartilage, there were no overall significant changes in the content and distribution of these molecules. There was, however, considerable individual variation in both distribution and content. Analyses indicated that there was a trend in osteoarthritic cartilage toward a loss of biglycan and decorin from the more superficial layers of intact cartilage, where both these molecules are normally more concentrated. This was accompanied by maintenance of proteoglycan content deeper in the cartilage, regardless of the degree of degeneration.
本研究旨在确定核心蛋白聚糖和双糖链蛋白聚糖在成人股骨髁软骨中的含量及分布，以及这些成分在骨关节炎中是否会发生变化。使用代表每种蛋白聚糖氨基末端21个氨基酸序列的肽（添加酪氨酸用于放射性碘化）和针对这两种分子产生的兔抗血清中的抗体，建立了新的放射免疫分析方法。用4M盐酸胍提取软骨以测定总含量，并通过色谱法分析提取物以确定分子大小。将冰冻切片平行于关节表面切割并进行提取，以确定其在组织内的分布。在解离条件下于琼脂糖CL - 2B上进行凝胶色谱分析，结果显示正常和骨关节炎软骨中均存在分配系数为0.7 - 0.75的分子。在正常成人软骨中，双糖链蛋白聚糖和核心蛋白聚糖核心蛋白的平均含量经计算分别约为每克湿重0.34毫克和0.48毫克。这些代表的摩尔含量与聚集蛋白聚糖相似。在骨关节炎软骨中，这些分子的含量和分布总体上没有显著变化。然而，在分布和含量方面均存在相当大的个体差异。分析表明，在骨关节炎软骨中存在一种趋势，即完整软骨较浅层的双糖链蛋白聚糖和核心蛋白聚糖会减少，而这两种分子通常在这些浅层更为集中。与此同时，无论退变程度如何，软骨深层的蛋白聚糖含量均保持不变。
