Habácek J, Braná H
Mol Gen Genet. 1976 Mar 22;144(2):217-21. doi: 10.1007/BF02428112.
In conjugation with donor strains carrying proximal F merogenotes of KLF-1 type about 100-fold lower frequency of Leu+ or Lac+ recombinants was found. The determination of the level of beta-galactosidase synthesis during the initial period of mating indicated that the transfer process of plasmid DNA was not impaired. Among the recombinants selected a large fraction have not expressed the plasmic fertility functions. This phenomenon was found to be replicon specific and was observed only with proximal F merogenotes but not with classical F'lac and F'ORF-1 elements or R1-19 plasmid. The expression of KLF-1 plasmid functions in the cell seems to be affected by a chromosomal gene of the proximal F merogenote closely linked to leu marker.
与携带KLF - 1型近端F因子的供体菌株接合时，发现亮氨酸+或乳糖+重组体的频率降低了约100倍。在交配初期对β-半乳糖苷酶合成水平的测定表明，质粒DNA的转移过程未受损害。在所选的重组体中，很大一部分没有表达质粒的育性功能。发现这种现象具有复制子特异性，仅在近端F因子中观察到，而在经典的F'lac和F'ORF - 1元件或R1 - 19质粒中未观察到。近端F因子的KLF - 1质粒功能在细胞中的表达似乎受到与亮氨酸标记紧密连锁的近端F因子染色体基因的影响。
