Yen K M, Gunsalus I C
Proc Natl Acad Sci U S A. 1982 Feb;79(3):874-8. doi: 10.1073/pnas.79.3.874.
Genes for naphthalene metabolism are localized on nah7, an 83-kilobase (kb) plasmid, in two gene clusters under salicylate control. Polar mutations formed by insertion of the transposon Tn5 permit detection of the transcription direction and the gene organization within two approximately 10-kb DNA segments separated by a approximately 7-kb regulatory gene region. The gene cluster specifying conversion of naphthalene to salicylate lies near the left initiation of a 25-kb DNA fragment A released by EcoRI; that for the salicylate pathway via catechol meta-fission lies near the right terminus with extension into the adjoining 5.9-kb fragment C. The genetic organization and regulation resemble the tol plasmid-encoded "upper" and "lower" pathways of toluene/xylene oxidation in Pseudomonas putida mt2.
萘代谢基因定位于nah7质粒上，该质粒大小为83千碱基对（kb），处于水杨酸控制下的两个基因簇中。由转座子Tn5插入形成的极性突变可用于检测转录方向以及两个约10 kb DNA片段内的基因组织，这两个片段被一个约7 kb的调控基因区域隔开。指定萘转化为水杨酸的基因簇位于由EcoRI释放的25 kb DNA片段A的左起始端附近；通过儿茶酚间位裂变的水杨酸途径的基因簇位于右末端附近，并延伸至相邻的5.9 kb片段C中。其遗传组织和调控类似于恶臭假单胞菌mt2中tol质粒编码的甲苯/二甲苯氧化的“上”、“下”途径。
