Gerber M R, Farrell A, Deshaies R J, Herskowitz I, Morgan D O
Department of Physiology, University of California, San Francisco 94143-0444, USA.
Proc Natl Acad Sci U S A. 1995 May 9;92(10):4651-5. doi: 10.1073/pnas.92.10.4651.
Studies of the temperature-sensitive cdc37-1 mutant of Saccharomyces cerevisiae suggest that Cdc37 is required for passage through the G1 phase of the cell cycle, but its precise function is not known. We have investigated the role of Cdc37 in the regulation of the cyclin-dependent protein kinase Cdc28. We find that G1 arrest in the cdc37-1 mutant is accompanied by a decrease in the Cdc28 activity associated with the G1 cyclin Cln2. This defect appears to be caused by a decrease in the binding of Cdc28 and Cln2. cdc37-1 mutants also exhibit a defect in the binding and activation of Cdc28 by the mitotic cyclin Clb2. Thus Cdc37 may be a regulator that is required for the association of Cdc28 with multiple cyclins.
对酿酒酵母温度敏感型cdc37 - 1突变体的研究表明，Cdc37是细胞周期G1期进程所必需的，但其确切功能尚不清楚。我们研究了Cdc37在细胞周期蛋白依赖性蛋白激酶Cdc28调控中的作用。我们发现，cdc37 - 1突变体中的G1期阻滞伴随着与G1期细胞周期蛋白Cln2相关的Cdc28活性降低。这种缺陷似乎是由Cdc28与Cln2结合减少所致。cdc37 - 1突变体在有丝分裂细胞周期蛋白Clb2对Cdc28的结合和激活方面也表现出缺陷。因此，Cdc37可能是Cdc28与多种细胞周期蛋白结合所必需的调节因子。
