Ridley A M
Laboratory of Microbiological Reagents (LMR), Central Public Health Laboratory, London.
Res Microbiol. 1995 Jan;146(1):21-34. doi: 10.1016/0923-2508(96)80268-3.
The results of an evaluation of restriction fragment length polymorphism (RFLP) typing for Listeria monocytogenes are presented. The method depends on the use of cloned DNA fragments from an L. monocytogenes (serovar 4b) strain to probe Southern blotted NciI restriction fragments derived from L. monocytogenes strains. Analyses of 862 isolates of serogroups 1/2, 3 and 4 were performed and a total of 32 RFLP patterns were observed. Interstrain RFLPs were common within serogroup 1/2 and the numerical index of discriminatory power for this group was 0.883. Serogroup 4 strains were divided into two major and three minor RFLP types, suggesting a high level of genotypic homogeneity, and the numerical index of discriminatory power was correspondingly low. The technique was found to be highly reproducible, and the stability of patterns was demonstrated by analyses of passaged strains and multiple isolates of the same strain from related specimens. RFLP typing is of value in epidemiological investigations involving strains of serogroup 1/2.
本文介绍了对单核细胞增生李斯特菌进行限制性片段长度多态性（RFLP）分型的评估结果。该方法依赖于使用来自单核细胞增生李斯特菌（血清型4b）菌株的克隆DNA片段，来探测源自单核细胞增生李斯特菌菌株的经Southern印迹的NciI限制性片段。对血清群1/2、3和4的862株分离株进行了分析，共观察到32种RFLP模式。血清群1/2内菌株间的RFLP很常见，该组的鉴别力数值指数为0.883。血清群4菌株分为两种主要和三种次要的RFLP类型，表明基因型同质性较高，相应地鉴别力数值指数较低。该技术具有高度可重复性，通过对传代菌株和来自相关标本的同一菌株的多个分离株进行分析，证明了模式的稳定性。RFLP分型在涉及血清群1/2菌株的流行病学调查中具有价值。
