Fekrat S, Wendel R T, de la Cruz Z, Green W R
Eye Pathology Laboratory, Wilmer Eye Institute, Baltimore, MD, USA.
Retina. 1995;15(1):53-7. doi: 10.1097/00006982-199515010-00010.
To describe the clinicopathologic features of an epiretinal membrane associated with a recurrent, full-thickness idiopathic macular hole and speculate on the mechanism(s) contributing to its recurrence 1 year after initially successful closure of pars plana vitrectomy and gas tamponade (SF6).
After fixation of the 2 x 1 mm specimen in a mixture of 1% glutaraldehyde and 4% formaldehyde followed by 2.5% glutaraldehyde, postfixation with osmium tetroxide, and standard dehydration, the specimen was embedded in epoxy resin. Ultrathin sections were stained with uranyl acetate and lead citrate for transmission electron microscopy.
Ultrastructural examination disclosed a fibrocellular membrane composed of Müller cells and fibrous astrocytes. Native collagen was entrapped in the matrix in some areas.
Those cells that may lead to the closure of an idiopathic macular hole may also contribute to its recurrence if the reparative process goes awry.
描述与复发性、全层特发性黄斑裂孔相关的视网膜前膜的临床病理特征，并推测在最初成功进行玻璃体切除术联合气体填塞（六氟化硫）封闭黄斑裂孔1年后导致其复发的机制。
将2×1毫米的标本固定于1%戊二醛和4%甲醛的混合液中，随后用2.5%戊二醛固定，再用四氧化锇后固定，经标准脱水后，将标本包埋于环氧树脂中。超薄切片用醋酸铀和枸橼酸铅染色，用于透射电子显微镜检查。
超微结构检查显示为一种由 Müller 细胞和纤维性星形胶质细胞组成的纤维细胞性膜。在某些区域，天然胶原蛋白被困于基质中。
那些可能导致特发性黄斑裂孔闭合的细胞，如果修复过程出现差错，也可能导致其复发。
